ABSTRACT
Objective:To simultaneously determine the contents of berberine hydroehloride and baicalin in Lanqin Oral Liquid via an HPLC method.Method:The HPLC with a Cl8 column(4.6?150 mm,5?m)was used.The mobile phases were as follows:the methanol-0.5%orthophosphoric acid(0～16.5 min,methanol 38%～40%,V=1 ml/min;16.8～18 min,methanol 99%,V=1.6ml/min;18.1～22min,methanol 38%,V=1.6ml/min;22.1～23 min,methanol 38%,V=1 ml/min.).The wavelength for detection was located at 275nm.Result:The linear ranges of berberine hydrochloride and baicallin were 6.8～65.28?g/ml and 31.2～299.52?g/ml,respectively,both showing a good linar relationship,and the average spotting recoveries were higher than 97.0%.The coefficient of variation was smaller than 2.0%.Con-clusion:The method is convenient and can be used for controlling the quality of Lanqin Oral Liquid.